9 Caillol M, Aioun J, Baly C, Persuy MA, Salesse R.Differential regulation of leptin receptor but not orexin in the hypothalamus of the lactating rat. Role for hypocretin in mediating stress-induced reinstatement of cocaine-seeking behavior. 7 Boutrel B, Kenny PJ, Specio SE, Martin-Fardon R, Markou A, Koob GF, de Lecea L.Cellular localization of orexins in human anterior pituitary. 6 Blanco M, Gallego R, Garcia-Caballero T, Dieguez C, Beiras A.Expression of gamma-aminobutyric acid B receptor subunits in hypothalamus of male and female developing rats. 5 Bianchi MS, Lux-Lantos VA, Bettler B, Libertun C.Selective action of orexin (hypocretin) on nonspecific thalamocortical projection neurons. 4 Bayer L, Eggermann E, Saint-Mleux B, Machard D, Jones BE, Muhlethaler M, Serafin M.Hypocretins (orexins) and sleep-wake disorders.
Distinct recognition of OX1 and OX2 receptors by orexin peptides.
2 Ammoun S, Holmqvist T, Shariatmadari R, Oonk HB, Detheux M, Parmentier M, Akerman KE, Kukkonen JP. GABA-mediated control of hypocretin- but not melanin-concentrating hormone-immunoreactive neurones during sleep in rats. 1 Alam MN, Kumar S, Bashir T, Suntsova N, Methippara MM, Szymusiak R, McGinty D. Here, we demonstrate that, in the physiological neuroendocrine condition leading to ovulation, information to the orexinergic system acts in hypothalamus and pituitary by different mechanisms. Reproduction, energy balance, and sleep-wake cycle are integrated. Cetrorelix or pentobarbital prevented increases of OX 1 and OX 2 only in the pituitary and blunted gonadotropin surges, but left OX 1, OX 2, and PPO brain expression unchanged. PPO in AH and MBH showed a fourfold or higher increase only during proestrus afternoon. OX 1 and OX 2 expression increased at least threefold in AH, MBH, and P, but not in CC, between 17 of proestrus, without variations in estrus, diestrus, or in males. Finally, OX 1, OX 2, and PPO were measured by real-time RT-PCR in tissues collected at 1900 of proestrus after treatments at 1400 with ovulation-blocking agents Cetrorelix or pentobarbital. Hormones and food consumption were determined. Main findings were confirmed and extended to all days of the cycle by quantitative real-time RT-PCR. Anterior (AH) and mediobasal (MBH) hypothalamus, anterior pituitary (P), and frontoparietal cortex (CC) were homogenized in TRIzol, and mRNAs were obtained for screening of PPO, OX 1, OX 2 expression by semiquantitative RT-PCR. Primarily, PPO, OX 1, and OX 2 expression was determined in Sprague-Dawley female cycling rats during proestrus and in males. We studied the expression of prepro-orexin (PPO), OX 1, and OX 2 in brain and pituitary under the influence of the hormonal status in adult rats. Orexins and their receptors OX 1 and OX 2 regulate energy balance and the sleep-wake cycle.
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